{"id":82649,"date":"2019-01-30T12:14:32","date_gmt":"2019-01-30T17:14:32","guid":{"rendered":"https:\/\/mrnf.gouv.qc.ca\/nos-publications\/rapds-aid-evaluate\/"},"modified":"2019-01-30T12:14:32","modified_gmt":"2019-01-30T17:14:32","slug":"rapds-aid-evaluate","status":"publish","type":"cpt_publication","link":"https:\/\/mrnf.gouv.qc.ca\/en\/our-publications\/rapds-aid-evaluate\/","title":{"rendered":"RAPDs as an aid to evaluate the genetic integrity of somatic embryogenesis-derived populations of Picea mariana (Mill.) B.S.P."},"content":{"rendered":"\n<p>Published in <strong>Theoretical and Applied Genetics 86: 81-87<\/strong><\/p>\n<p>The usefulness of random amplified polymorphic DNA (RAPD) in assessing the genetic stability of somatic embryogenesis-derived populations of black spruce [<em>Picea mariana<\/em> (Mill.) B.S.P.] was evaluated. Three arbitrary 11-mer primers were successfully used to amplify DNA from both in-vivo and in-vitro material. Twenty-five embryogenic cell lines, additional zygotic embryos and megagametophytes from three controlled crosses involving four selected genotypes of black spruce were used for the segregation analysis of RAPD variants. Ten markers were genetically characterized and used to evaluate the genetic stability of somatic embryos derived from three embryogenic cell lines (one cell line per cross, 30 somatic embryos per cell line). No variation was detected within clones. The utilization of RAPD markers both for the assessment of genetic stability of clonal materials and to certify genetic stability throughout the process of somatic embryogenesis is discussed.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Published in Theoretical and Applied Genetics 86: 81-87 The usefulness of random amplified polymorphic DNA (RAPD) in assessing the genetic stability of somatic embryogenesis-derived populations of black spruce [Picea mariana (Mill.) B.S.P.] was evaluated. Three arbitrary 11-mer primers were successfully used to amplify DNA from both in-vivo and in-vitro material. Twenty-five embryogenic cell lines, additional [&hellip;]<\/p>\n","protected":false},"template":"","format":"standard","cptt_secteur":[1179],"cptt_theme":[1180,1181,1187],"cptt_categ_publi":[1149],"cptt_auteurs_ministeriels":[],"class_list":["post-82649","cpt_publication","type-cpt_publication","status-publish","format-standard","hentry","cptt_secteur-forests","cptt_theme-forestry-research","cptt_theme-forests","cptt_theme-seeds-and-forest-seedlings-production","cptt_categ_publi-scientific-article"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v24.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>RAPDs as an aid to evaluate the genetic integrity of somatic embryogenesis-derived populations of Picea mariana (Mill.) B.S.P. - Minist\u00e8re des Ressources naturelles et des For\u00eats<\/title>\n<meta name=\"description\" content=\"The usefulness of random amplified polymorphic DNA in assessing the genetic stability of somatic embryogenesis-derived populations.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mrnf.gouv.qc.ca\/en\/our-publications\/rapds-aid-evaluate\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"RAPDs as an aid to evaluate the genetic integrity of somatic embryogenesis-derived populations of Picea mariana (Mill.) B.S.P. - Minist\u00e8re des Ressources naturelles et des For\u00eats\" \/>\n<meta property=\"og:description\" content=\"The usefulness of random amplified polymorphic DNA in assessing the genetic stability of somatic embryogenesis-derived populations.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/mrnf.gouv.qc.ca\/en\/our-publications\/rapds-aid-evaluate\/\" \/>\n<meta property=\"og:site_name\" content=\"Minist\u00e8re des Ressources naturelles et des For\u00eats\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/RessourcesNaturellesForets\/\" \/>\n<meta property=\"og:image\" content=\"\/wp-content\/uploads\/Image-g%c3%a9n%c3%a9rale-hiver_FB.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1200\" \/>\n\t<meta property=\"og:image:height\" content=\"630\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:site\" content=\"@mrnfqc\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/mrnf.gouv.qc.ca\/en\/our-publications\/rapds-aid-evaluate\/\",\"url\":\"https:\/\/mrnf.gouv.qc.ca\/en\/our-publications\/rapds-aid-evaluate\/\",\"name\":\"RAPDs as an aid to evaluate the genetic integrity of somatic embryogenesis-derived populations of Picea mariana (Mill.) B.S.P. - Minist\u00e8re des Ressources naturelles et des For\u00eats\",\"isPartOf\":{\"@id\":\"https:\/\/mrnf.gouv.qc.ca\/#website\"},\"datePublished\":\"2019-01-30T17:14:32+00:00\",\"description\":\"The usefulness of random amplified polymorphic DNA in assessing the genetic stability of somatic embryogenesis-derived populations.\",\"breadcrumb\":{\"@id\":\"https:\/\/mrnf.gouv.qc.ca\/en\/our-publications\/rapds-aid-evaluate\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/mrnf.gouv.qc.ca\/en\/our-publications\/rapds-aid-evaluate\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/mrnf.gouv.qc.ca\/en\/our-publications\/rapds-aid-evaluate\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Accueil\",\"item\":\"https:\/\/mrnf.gouv.qc.ca\/en\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Publications\",\"item\":\"https:\/\/mrnf.gouv.qc.ca\/nos-publications\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"RAPDs as an aid to evaluate the genetic integrity of somatic embryogenesis-derived populations of Picea mariana (Mill.) B.S.P.\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/mrnf.gouv.qc.ca\/#website\",\"url\":\"https:\/\/mrnf.gouv.qc.ca\/\",\"name\":\"Minist\u00e8re des Ressources naturelles et des For\u00eats\",\"description\":\"Prototype WordPress\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/mrnf.gouv.qc.ca\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"RAPDs as an aid to evaluate the genetic integrity of somatic embryogenesis-derived populations of Picea mariana (Mill.) B.S.P. - Minist\u00e8re des Ressources naturelles et des For\u00eats","description":"The usefulness of random amplified polymorphic DNA in assessing the genetic stability of somatic embryogenesis-derived populations.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/mrnf.gouv.qc.ca\/en\/our-publications\/rapds-aid-evaluate\/","og_locale":"en_US","og_type":"article","og_title":"RAPDs as an aid to evaluate the genetic integrity of somatic embryogenesis-derived populations of Picea mariana (Mill.) B.S.P. - Minist\u00e8re des Ressources naturelles et des For\u00eats","og_description":"The usefulness of random amplified polymorphic DNA in assessing the genetic stability of somatic embryogenesis-derived populations.","og_url":"https:\/\/mrnf.gouv.qc.ca\/en\/our-publications\/rapds-aid-evaluate\/","og_site_name":"Minist\u00e8re des Ressources naturelles et des For\u00eats","article_publisher":"https:\/\/www.facebook.com\/RessourcesNaturellesForets\/","og_image":[{"width":1200,"height":630,"url":"\/wp-content\/uploads\/Image-g%c3%a9n%c3%a9rale-hiver_FB.jpg","type":"image\/jpeg"}],"twitter_card":"summary_large_image","twitter_site":"@mrnfqc","twitter_misc":{"Est. reading time":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/mrnf.gouv.qc.ca\/en\/our-publications\/rapds-aid-evaluate\/","url":"https:\/\/mrnf.gouv.qc.ca\/en\/our-publications\/rapds-aid-evaluate\/","name":"RAPDs as an aid to evaluate the genetic integrity of somatic embryogenesis-derived populations of Picea mariana (Mill.) B.S.P. - Minist\u00e8re des Ressources naturelles et des For\u00eats","isPartOf":{"@id":"https:\/\/mrnf.gouv.qc.ca\/#website"},"datePublished":"2019-01-30T17:14:32+00:00","description":"The usefulness of random amplified polymorphic DNA in assessing the genetic stability of somatic embryogenesis-derived populations.","breadcrumb":{"@id":"https:\/\/mrnf.gouv.qc.ca\/en\/our-publications\/rapds-aid-evaluate\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/mrnf.gouv.qc.ca\/en\/our-publications\/rapds-aid-evaluate\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/mrnf.gouv.qc.ca\/en\/our-publications\/rapds-aid-evaluate\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Accueil","item":"https:\/\/mrnf.gouv.qc.ca\/en\/"},{"@type":"ListItem","position":2,"name":"Publications","item":"https:\/\/mrnf.gouv.qc.ca\/nos-publications\/"},{"@type":"ListItem","position":3,"name":"RAPDs as an aid to evaluate the genetic integrity of somatic embryogenesis-derived populations of Picea mariana (Mill.) B.S.P."}]},{"@type":"WebSite","@id":"https:\/\/mrnf.gouv.qc.ca\/#website","url":"https:\/\/mrnf.gouv.qc.ca\/","name":"Minist\u00e8re des Ressources naturelles et des For\u00eats","description":"Prototype WordPress","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/mrnf.gouv.qc.ca\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"}]}},"_links":{"self":[{"href":"https:\/\/mrnf.gouv.qc.ca\/en\/wp-json\/wp\/v2\/cpt_publication\/82649","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mrnf.gouv.qc.ca\/en\/wp-json\/wp\/v2\/cpt_publication"}],"about":[{"href":"https:\/\/mrnf.gouv.qc.ca\/en\/wp-json\/wp\/v2\/types\/cpt_publication"}],"version-history":[{"count":0,"href":"https:\/\/mrnf.gouv.qc.ca\/en\/wp-json\/wp\/v2\/cpt_publication\/82649\/revisions"}],"wp:attachment":[{"href":"https:\/\/mrnf.gouv.qc.ca\/en\/wp-json\/wp\/v2\/media?parent=82649"}],"wp:term":[{"taxonomy":"cptt_secteur","embeddable":true,"href":"https:\/\/mrnf.gouv.qc.ca\/en\/wp-json\/wp\/v2\/cptt_secteur?post=82649"},{"taxonomy":"cptt_theme","embeddable":true,"href":"https:\/\/mrnf.gouv.qc.ca\/en\/wp-json\/wp\/v2\/cptt_theme?post=82649"},{"taxonomy":"cptt_categ_publi","embeddable":true,"href":"https:\/\/mrnf.gouv.qc.ca\/en\/wp-json\/wp\/v2\/cptt_categ_publi?post=82649"},{"taxonomy":"cptt_auteurs_ministeriels","embeddable":true,"href":"https:\/\/mrnf.gouv.qc.ca\/en\/wp-json\/wp\/v2\/cptt_auteurs_ministeriels?post=82649"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}