{"id":82331,"date":"2019-01-30T12:11:58","date_gmt":"2019-01-30T17:11:58","guid":{"rendered":"https:\/\/mrnf.gouv.qc.ca\/nos-publications\/growth-ectomycorrhiza-containerized-black-spruce-seedlings-nitrogen-fertilization\/"},"modified":"2019-01-30T12:11:58","modified_gmt":"2019-01-30T17:11:58","slug":"growth-ectomycorrhiza-containerized-black-spruce-seedlings-nitrogen-fertilization","status":"publish","type":"cpt_publication","link":"https:\/\/mrnf.gouv.qc.ca\/en\/our-publications\/growth-ectomycorrhiza-containerized-black-spruce-seedlings-nitrogen-fertilization\/","title":{"rendered":"Growth and ectomycorrhiza formation of containerized black spruce seedlings as affected by nitrogen fertilization, Inoculum type, and symbiont"},"content":{"rendered":"\n<p>Published in <strong>Canadian Journal of Forest Research 18: 922-929<\/strong><\/p>\n<p><em>Picea mariana <\/em>(Mill.) B.S.P. seedlings were grown in containers under three levels of nitrogen fertilization (6, 8.5, and 15 mg\/seedling per season). Seedlings were inoculated at sowing with <em>Laccaria bicolor <\/em>and <em>Hebeloma cylindrosporum<\/em>, using two methods: mycelial suspension liquid inoculum and peat-vermiculite solid inoculum. Results show that liquid inoculum was as effective as solid inoculum for development of <em>Laccaria bicolor <\/em>ectomycorrhizae on black spruce seedlings. After 20 weeks in the greenhouse, seedlings fertilized with 8.5 mg N and inoculated with <em>L. bicolor <\/em>had the highest percentage of ectomycorrhizal short roots for both inoculum types, with 59 and 53% for liquid and solid inocula, respectively. These <em>L. bicolor <\/em>seedlings had significantly more ectomycorrhizae with 8.5 mg N than with 15 mg N. Very few ectomycorrhizae were obtained with <em>Hebeloma cylindrosporum <\/em>for any of the nitrogen levels or either of the inoculum types. There were no significant differences for any growth parameters except shoot\/root ratio between <em>L. bicolor <\/em>seedlings and the controls, regardless of the N level and inoculum type. For both inoculum types, only shoot height of seedlings differed significantly among the three N levels. Seedlings fertilized with 8.5 mg N had root-collar diameter and dry weights that were not significantly less than those that received 15 mg N for both inoculum types. It is only at the lower level of 6 mg N that seedlings inoculated with liquid <em>L. bicolor <\/em>inoculum absorbed significantly more N than the controls. Analysis of percent N and P concentration in seedlings with the greatest ectomycorrhizal development and analysis of available N and P in their substrate indicated that tissue concentrations of 1.9% N and 0.2% P and substrate fertility of 40 ppm N and 50 ppm P would be appropriate to maintain the ectomycorrhizal association <em>Picea mariana <\/em>&#8211; <em>Laccaria bicolor<\/em>. Salinity in the substrate of control seedlings was generally significantly different between the three N levels during the growing period.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Published in Canadian Journal of Forest Research 18: 922-929 Picea mariana (Mill.) B.S.P. seedlings were grown in containers under three levels of nitrogen fertilization (6, 8.5, and 15 mg\/seedling per season). Seedlings were inoculated at sowing with Laccaria bicolor and Hebeloma cylindrosporum, using two methods: mycelial suspension liquid inoculum and peat-vermiculite solid inoculum. Results show [&hellip;]<\/p>\n","protected":false},"template":"","format":"standard","cptt_secteur":[1179],"cptt_theme":[1180,1181,1187],"cptt_categ_publi":[1149],"cptt_auteurs_ministeriels":[1084],"class_list":["post-82331","cpt_publication","type-cpt_publication","status-publish","format-standard","hentry","cptt_secteur-forests","cptt_theme-forestry-research","cptt_theme-forests","cptt_theme-seeds-and-forest-seedlings-production","cptt_categ_publi-scientific-article","cptt_auteurs_ministeriels-j-gagnon"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v24.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Growth and ectomycorrhiza formation of containerized black spruce seedlings as affected by nitrogen fertilization, Inoculum type, and symbiont - Minist\u00e8re des Ressources naturelles et des For\u00eats<\/title>\n<meta name=\"description\" content=\"Article about growth and ectomycorrhiza formation of containerized black spruce seedlings as affected by nitrogen fertilization...\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mrnf.gouv.qc.ca\/en\/our-publications\/growth-ectomycorrhiza-containerized-black-spruce-seedlings-nitrogen-fertilization\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Growth and ectomycorrhiza formation of containerized black spruce seedlings as affected by nitrogen fertilization, Inoculum type, and symbiont - 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