{"id":82299,"date":"2019-01-30T12:14:32","date_gmt":"2019-01-30T17:14:32","guid":{"rendered":"https:\/\/mrnf.gouv.qc.ca\/nos-publications\/frequency-somaclonal-variation\/"},"modified":"2019-01-30T12:14:32","modified_gmt":"2019-01-30T17:14:32","slug":"frequency-somaclonal-variation","status":"publish","type":"cpt_publication","link":"https:\/\/mrnf.gouv.qc.ca\/en\/our-publications\/frequency-somaclonal-variation\/","title":{"rendered":"Frequency of somaclonal variation in plants of black spruce (Picea mariana, Pinaceae) and white spruce (P. glauca, Pinaceae) derived from somatic embryogenesis and identification of some factors involved in genetic instability"},"content":{"rendered":"\n<p>Published in <strong>American Journal of Botany 86(10): 1373-1381<\/strong><\/p>\n<p>Plants of black spruce (<em>Picea mariana, N<\/em> = 7047 individuals) and white spruce (<em>P. glauca, N<\/em> = 3995 individuals) were regenerated from a total of 87 clones over a 5-yr period by somatic embryogenesis to study factors that might be associated with the appearance of variant phenotypes. Morphological evaluation of the plants showed several types of variation. These variations were grouped into nine types: dwarfism (type A), reduced height with various form anomalies (types B, C, and D), needle fasciation (type E), abnormality in tree architecture (type F), <em>variegata<\/em> phenotype (type G), and plants with an overall regular morphology but smaller than normal plants (type H). Plagiotropic plants were also observed (type I). Each plant from types A to H (except type C where no plants survived more than 6 mo) had retained its phenotype over 4-5 yr of growth. Some of the variant types could be related to chromosomic instability: chromosome counts showed aneuploid cells for type-A and type-D plants. The type I (plagiotropism) was not related to genetic instability but rather to physiological disorders. In total, spruce variants of types A-H were obtained at relatively low frequencies, i.e., 1.0% (39\/3995) for white spruce and 1.6% (110\/7047) for black spruce. Statistical analyses, conducted with family, clone, and time in maintenance as variables, showed that clone was the most important source of genetic instability followed by time in maintenance.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Published in American Journal of Botany 86(10): 1373-1381 Plants of black spruce (Picea mariana, N = 7047 individuals) and white spruce (P. glauca, N = 3995 individuals) were regenerated from a total of 87 clones over a 5-yr period by somatic embryogenesis to study factors that might be associated with the appearance of variant phenotypes. [&hellip;]<\/p>\n","protected":false},"template":"","format":"standard","cptt_secteur":[1179],"cptt_theme":[1180,1181,1187],"cptt_categ_publi":[1149],"cptt_auteurs_ministeriels":[],"class_list":["post-82299","cpt_publication","type-cpt_publication","status-publish","format-standard","hentry","cptt_secteur-forests","cptt_theme-forestry-research","cptt_theme-forests","cptt_theme-seeds-and-forest-seedlings-production","cptt_categ_publi-scientific-article"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v24.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Frequency of somaclonal variation in plants of black spruce (Picea mariana, Pinaceae) and white spruce (P. glauca, Pinaceae) derived from somatic embryogenesis and identification of some factors involved in genetic instability - Minist\u00e8re des Ressources naturelles et des For\u00eats<\/title>\n<meta name=\"description\" content=\"Plants of black spruce and white spruce were regenerated from a total of 87 clones over a 5-yr period by somatic embryogenesis\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mrnf.gouv.qc.ca\/en\/our-publications\/frequency-somaclonal-variation\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Frequency of somaclonal variation in plants of black spruce (Picea mariana, Pinaceae) and white spruce (P. glauca, Pinaceae) derived from somatic embryogenesis and identification of some factors involved in genetic instability - 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