{"id":82052,"date":"2019-01-30T12:14:32","date_gmt":"2019-01-30T17:14:32","guid":{"rendered":"https:\/\/mrnf.gouv.qc.ca\/nos-publications\/carbohydrate-requirements-development-black-spruce\/"},"modified":"2019-01-30T12:14:32","modified_gmt":"2019-01-30T17:14:32","slug":"carbohydrate-requirements-development-black-spruce","status":"publish","type":"cpt_publication","link":"https:\/\/mrnf.gouv.qc.ca\/en\/our-publications\/carbohydrate-requirements-development-black-spruce\/","title":{"rendered":"Carbohydrate requirements for the development of black spruce (Picea mariana (Mill.) B.S.P.) and red spruce (P. rubens Sarg.) somatic embryos"},"content":{"rendered":"\n<p>Published in <strong>Plant Cell, Tissue and Organ Culture 27: 95-103<\/strong><\/p>\n<p>Different carbohydrates were investigated for somatic embryo development of black spruce and red spruce. They were tested in a basal maturation medium consisting of Litvay&#8217;s salts at half-strength containing 1 g l<sup>-1<\/sup> glutamine, 1 g l<sup>-1<\/sup> casein hydrolysate, 7.5 <em>m<\/em>M abscisic acid, and 0.9% Difco Bacto-agar. A comparison of different sucrose concentrations showed that 6% was optimal for embryo development. Among the nine carbohydrates tested, sucrose, fructose, glucose, maltose, and cellobiose supported embryo development while arabinose, mannitol, myo-inositol, and sorbitol did not. A comparison of sucrose, glucose, and fructose at three concentrations showed that the general pattern of response for both species followed concentration expressed as a percentage, independent of the molarity of carbohydrate in the medium. Interspecific differences were observed concerning carbohydrate requirements. For red spruce, 6% fructose was found best for embryo development, while no such preference was observed for black spruce. No significant difference was observed in the number of embryos produced with 6% sucrose or 3% sucrose plus an equimolar concentration of either mannitol, sorbitol, or myo-inositol in the maturation medium, suggesting that the effect of the carbohydrate on the maturation was partly osmotic.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Published in Plant Cell, Tissue and Organ Culture 27: 95-103 Different carbohydrates were investigated for somatic embryo development of black spruce and red spruce. They were tested in a basal maturation medium consisting of Litvay&#8217;s salts at half-strength containing 1 g l-1 glutamine, 1 g l-1 casein hydrolysate, 7.5 mM abscisic acid, and 0.9% Difco [&hellip;]<\/p>\n","protected":false},"template":"","format":"standard","cptt_secteur":[1179],"cptt_theme":[1180,1181,1187],"cptt_categ_publi":[1149],"cptt_auteurs_ministeriels":[],"class_list":["post-82052","cpt_publication","type-cpt_publication","status-publish","format-standard","hentry","cptt_secteur-forests","cptt_theme-forestry-research","cptt_theme-forests","cptt_theme-seeds-and-forest-seedlings-production","cptt_categ_publi-scientific-article"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v24.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Carbohydrate requirements for the development of black spruce (Picea mariana (Mill.) B.S.P.) and red spruce (P. rubens Sarg.) somatic embryos - Minist\u00e8re des Ressources naturelles et des For\u00eats<\/title>\n<meta name=\"description\" content=\"Different carbohydrates were investigated for somatic embryo development of black spruce and red spruce.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/mrnf.gouv.qc.ca\/en\/our-publications\/carbohydrate-requirements-development-black-spruce\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Carbohydrate requirements for the development of black spruce (Picea mariana (Mill.) 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